Role Of Enteropathogenic Escherichia Coli In Paediatric Diarrhoeas In South India
Role Of Enteropathogenic Escherichia Coli In Paediatric Diarrhoeas
Keywords:
Andhra Pradesh , Enteropathogenic Escherichia coli ( EPEC) , Antibiotic Susceptibility , Serotyping , Molecular characterisation , Enteroaggregative Escherchia coli ( EAEC)Abstract
Background: Enteropathogenic Escherichia coli( EPEC) is a major cause of diarrhoea in children below 5 years of age. Serotyping is the classical method and PCR detection of virulence factors is a rapid way of detecting diarrhoeagenic Esch.coli. Objectives : To study the role of EPEC in Paediatric diarrhoea by both Serogrouping and Multiplex PCR assay and to analyse the antibiotic susceptibililty pattern of EPEC strains in our area. Materials and Methods : Prospective study of stool samples collected from children with diarrhoea and without diarrhoea who were below 5 years of age was conducted from May to November 2011. EPEC isolates were identified by Serogrouping. Escherichia coli isolates were subjected to Serogrouping and Multiplex PCR assay and those isolates which showed pathogenic genes were further serotyped. Antibiotic susceptibility pattern of EPEC isolates was determined by Clinical and Laboratory Standards Institute guidelines. Results : Among the Escherichia coli isolates in the diarrhoeal group, 36.8% were identified as EPEC by Serogrouping and 38.8% of them were found to possess EAEC genes by molecular characterisation. In the nondiarrhoeal Esch. coli strains , none agglutinated with EPEC polyvalent sera, 46.6% showed EAEC genes out of which 85.7% were of a single serotype O153. Among the Escherichia coli isolates which agglutinated with EPEC polyvalent antisera , 33.3% were positive for Enteroaggregative genes. Conclusion : EPEC is still an important pathogen in paediatric diarrhoeas . O serogrouping can still be relied upon for detection of EPEC. EAEC are present in classical ‘ O ‘ serogroups. Serotype O 153 has an increasing potential for asymptomatic carrier state in children below 5 years of age.
References
1. Mamatha B , Shivananda P.G. Rotavirus and Enteric pathogens in Infectious Diarrhoea in Manipal , South India . Indian Journal of Pediatrics 2002 ; 69 (5) : 393 - 396
2. Samuel .V , Daniel R, Margarite P. Prevalence of diarrheagenic Esch.coli in children from Leon , Nicaragua . Joural of Medical Microbiology 2009 ; 58(5) : 630 – 637
3. Ashraful Haq J , Hirchoon L , Rosliza Abdur R Detection of Enteropathogebnic Esch.coli by serotyping & cell AdhesionAssay among children in N.Eastern Peninsular Malaysia : A Hospital Based study . Ibrahim Medical College .J. 2008 ; 2(2) : 40 – 43
4. Hala A.R.E , Hoda A.H.I , Marwa N.E – Multiplex PCR for Detection of Diarrhoeagenic Esch.coli in Egyptian children . Journal of Medical Sciences 2007 , 7:255-262
5. Neelam T,Balvinder M , Sumeeta.K . Antimicrobial resistance in selected bacterial enteropathogens in N.India . Indian J Med Res 2004 ; 120 : 39 – 43
6. Arif A , Asiya A , Zahida Q . Drug resistance in Indian isolates of enteropathogenic Esch.coli. World J Microbial Biotechmol 2008 , 24 : 2633-2638
7. Joshi CK, Bhardwaj AK , Vyas BL. A Study of bacterial infantile diarrhoea, India J Pediat 1980, Vol. 47; p. 307 – 310.
8. Khanna KK, Ramanathan AL, Puri RK , Bhatia VN and Sundaraj T. Childhood Diarrhoea and its Association with Enteropathogenic Esch.coli “, Indian Journal of Pediatrics Jully 1977, Vol. 44, 354, P. 169 – 175.
9. Amela D , Mirsada H , Daria K. Diarrhoeagenic Esch.coli strains isolated from pediatric patients with Diarrhoea in Bosnia and Herzegonia. Bousman Journal of Basic Medical Survices 2009 ; 9 (2) : 148 -155.
10. Prasannan M , Jesudason MV , Kang G. A study on some phenotypic virulence markers of Enteropathogenic Esch.coli. Indian J Med Res.2004 ; 114 : 95 - 8
11. Tawfeek H.I , Najim N.H , N.Mashikhi S. Studies on diarrhoeal illness among hospitalised children under 5 years of age in Baghdad during 1990-97. 2002 ; 8 (1)
12. Addy PAK, Antepim G, Frimpong EH . “ Prevalence of pathogenic Escherichia coli and parasites in infants with Diarrhoea in kumasi , Ghana “ , East African Medical Journal Nweze E.I Aetiology of Diarrhoea & virulance properties of diarrhoeagenic Esch.coli among patients & Healthy
subjects in S.E Nigeria. J Heath Popul Nutr 2010 ; 28 ( 3) : 245 – 252
13. Alikhani M , Mirsalahian A , Fatollahadeh B. Prevalence of Enteropathogenic & Shiga toxin producing Esch.coli among children with & without Diarrhoea in Iran . Journal of Health, Population and Nutrition . ( J Health popul Nutr , 2007 ; 25 (1) ; 88-93
14. Nataro JP,Kaper JB , Diarrhoeagenic Esch.coli Clinical Microbiology Rev.1998 ; 11: 142-201
, 2004 , Vol, No.7 , p. 1-2
15. Prasannan M, Jesudason MV , Kang G, Sridharan G.A Study on some phenotypic virulance markers of Enteropathogenic Esch.coli , Indian J Med Res. September , 2001; Vol 114: 91 - 95
16. El-Gilamy A.H , Hanmool S. Epidemiology of diarrhoeal diseases among children under age 5 years in Dakahlia , Egypt. Eastern Mediterronean Health Journal , 2005 ; 11 ( 4) 762 – 775.
17. Cristiane Barros C , solange Barros C , Magda Maria S . Early aquisition of serum & saliva antibiotics reactive to enteropathogenic Esch.coli virulance associated proteins by infants living in an endemic area.Pediatric Allergy and Immunology 2003 ; 14 ( 3) : 222 – 228.
18. Barabara J S , Roger I G , Imdaul H M, Khan MU , James E H , Hasina B . Surveillance of patients attending a diarrhoeal disease hospital in Bangladesh . British Medical Journal , 23 October , 1982, Vol.285, p. 1185 – 1188.
19. Ulysses Fagundes-Neto , Isabel Cristina Affonso Scarletsky . The gut at war : the consequences of enteropathogenic Esch.coli infection as a factor of Diarrhoea and malnutrition , Sao Paulo Med J/ Rev. Paul Med. 2000 ; 118 (1) , p. 21 – 29
20. Enterobacterial infections . Encyclopedia of Childrens Health 2005 edition
21. Mercado E , Ochoa , T , Ecker L , ckary T. Fecal leucocytes in children infected with diarrhoeagenic Esch.coli Jour nal of clinical Microbiology 2011 ; 49 (4) : 1376 – 138122. Saxena SN , Mago ML , Rao Bhanu LN , Ahuja S, Gowal D “ Prevalence of multiple drug resistance amongst starins of Esch.coli isolates from cases of Diarrhoeaâ€, Indian J pediat , 1983, 50: p. 607 – 611.
23. Djie – Maletz A , Reither K. Donours High rate of resistance to locally used antibiotics among enteric bacteria from children in Northern Ghana. J Antimicrob chemother 2008 ; 61, 1315 – 1318.
24. Trung Vu N , Phung Van L , Chinh Huy L . Antibiotic Resistance in Diarrhoeagenic Esch.coli and shigella strains isolated from children in Hanoi , Vietnam . Antimicrob agents Chemother 2005 ; 49 (2) : 816 – 819.
25. Daniel R , Luciand R , Nanci S. Antibiotic for emperical treatment of acute infections diarrhoea in children Braz J In Fect Dis 2006 ; 10 (3)
26. Kyung Hee Kim, Inn-Soo such et al. Etiology of Childhood Diarrhoea in Korea . Jr.of Clinical microbiology , 1989; 1192 – 96.
27. Teresa Estrada-Garcia ,Jorge F, Cerna C. “ Drug-resistant Diarrhoeagenic Esch.coli , Maxico “, Emerging Infectious Diseases , August , 2005 ; P 1-5
28. Jav Sarantuya , Junichiro Nishi , Naoko Wakimoto et al .Typical Enteroaggregative Escherichia coli is the Most Pre valent pathotype among E.coli Strains Causing Diarrhoea in Mangolian Children “Journal of Clinical Microbiology , January , 2004 Vol, 42, No. 1 ; p.133 – 139.
29. Souman K , Bharwati S , Rajendran K. Virulance characteristics and Molecular Epidemiology of Enteroaggregative Esch.coli isolates from Hospitalised diarrhoeal patients in Kolkata , India . J clin Microbiol 2004 ; 42 (9).
30. Uber AP, Trabulsi LR , Irino K . Enteroaggregative Esch.coli from humans and animals differ in major phenotypic traits and virulence genes. FEMS Microbiology Letters 2006 ; 256 ( 2 ) : 251 – 7.
31. Andrej wein traub. Enteroaggregative Esch.coli Epidemiology , virulance and detection , Jornal of Medical Microbiology 2007 ; 56(1) : 4 – 8. Vaishnavi C , Kaur SS Beutin L Krueger W Phenotypic and molecular characterisation of clinically isolated Esch.coli Indian Journal of Pathology & Microbiology 2010 ; 53 (3) : 503 – 508.
32. Angela Cristina Rodrigues G . Production of cytolethal distending toxin and other virulence characteristics of Escherichia coli Strains of serogroup 086. Mem.Inst . Oswaldo Cruz 2001 ; 96 : 703 – 8 .
33. Vaishnavi C , Kaur S , Beutin L . Phenotypic and molecular Characterisation of clinically isolated Escherichia coli Indian Journal of Pathology & Microbiology 2010 ; 53 ( 3) : 503 – 508.
34. Priyanka S and Alka P . Isolation of Esch.coli , staphylococcus aureus and Listeria monocytogenes from milk products sold under market conditions at AGRA REGION , Acta agriculture Slovenic 2008 ; 92 (1) : 83 – 88.
35. Waldir P. Samar F , Cristiano G . Enteropathogenic Esch.coli strains among classical Enteropathogenic Esch.coli O serogroups Journal of clinical Microbiology 2002 ; 40 ( 9 ) : 3540 – 3541
36. Leila C.campos , Maria R franzolui , Leiz R Trabulsi “ Diarrhoeagenic Esch.coli categories among the Traditional Enteropathogenic Esch.coli O serogroups - A Review Mem Inst Oswaldo Cruz, Rio De Janeiro , October 2004 ; 99 ( 6) : 545 – 552.
2. Samuel .V , Daniel R, Margarite P. Prevalence of diarrheagenic Esch.coli in children from Leon , Nicaragua . Joural of Medical Microbiology 2009 ; 58(5) : 630 – 637
3. Ashraful Haq J , Hirchoon L , Rosliza Abdur R Detection of Enteropathogebnic Esch.coli by serotyping & cell AdhesionAssay among children in N.Eastern Peninsular Malaysia : A Hospital Based study . Ibrahim Medical College .J. 2008 ; 2(2) : 40 – 43
4. Hala A.R.E , Hoda A.H.I , Marwa N.E – Multiplex PCR for Detection of Diarrhoeagenic Esch.coli in Egyptian children . Journal of Medical Sciences 2007 , 7:255-262
5. Neelam T,Balvinder M , Sumeeta.K . Antimicrobial resistance in selected bacterial enteropathogens in N.India . Indian J Med Res 2004 ; 120 : 39 – 43
6. Arif A , Asiya A , Zahida Q . Drug resistance in Indian isolates of enteropathogenic Esch.coli. World J Microbial Biotechmol 2008 , 24 : 2633-2638
7. Joshi CK, Bhardwaj AK , Vyas BL. A Study of bacterial infantile diarrhoea, India J Pediat 1980, Vol. 47; p. 307 – 310.
8. Khanna KK, Ramanathan AL, Puri RK , Bhatia VN and Sundaraj T. Childhood Diarrhoea and its Association with Enteropathogenic Esch.coli “, Indian Journal of Pediatrics Jully 1977, Vol. 44, 354, P. 169 – 175.
9. Amela D , Mirsada H , Daria K. Diarrhoeagenic Esch.coli strains isolated from pediatric patients with Diarrhoea in Bosnia and Herzegonia. Bousman Journal of Basic Medical Survices 2009 ; 9 (2) : 148 -155.
10. Prasannan M , Jesudason MV , Kang G. A study on some phenotypic virulence markers of Enteropathogenic Esch.coli. Indian J Med Res.2004 ; 114 : 95 - 8
11. Tawfeek H.I , Najim N.H , N.Mashikhi S. Studies on diarrhoeal illness among hospitalised children under 5 years of age in Baghdad during 1990-97. 2002 ; 8 (1)
12. Addy PAK, Antepim G, Frimpong EH . “ Prevalence of pathogenic Escherichia coli and parasites in infants with Diarrhoea in kumasi , Ghana “ , East African Medical Journal Nweze E.I Aetiology of Diarrhoea & virulance properties of diarrhoeagenic Esch.coli among patients & Healthy
subjects in S.E Nigeria. J Heath Popul Nutr 2010 ; 28 ( 3) : 245 – 252
13. Alikhani M , Mirsalahian A , Fatollahadeh B. Prevalence of Enteropathogenic & Shiga toxin producing Esch.coli among children with & without Diarrhoea in Iran . Journal of Health, Population and Nutrition . ( J Health popul Nutr , 2007 ; 25 (1) ; 88-93
14. Nataro JP,Kaper JB , Diarrhoeagenic Esch.coli Clinical Microbiology Rev.1998 ; 11: 142-201
, 2004 , Vol, No.7 , p. 1-2
15. Prasannan M, Jesudason MV , Kang G, Sridharan G.A Study on some phenotypic virulance markers of Enteropathogenic Esch.coli , Indian J Med Res. September , 2001; Vol 114: 91 - 95
16. El-Gilamy A.H , Hanmool S. Epidemiology of diarrhoeal diseases among children under age 5 years in Dakahlia , Egypt. Eastern Mediterronean Health Journal , 2005 ; 11 ( 4) 762 – 775.
17. Cristiane Barros C , solange Barros C , Magda Maria S . Early aquisition of serum & saliva antibiotics reactive to enteropathogenic Esch.coli virulance associated proteins by infants living in an endemic area.Pediatric Allergy and Immunology 2003 ; 14 ( 3) : 222 – 228.
18. Barabara J S , Roger I G , Imdaul H M, Khan MU , James E H , Hasina B . Surveillance of patients attending a diarrhoeal disease hospital in Bangladesh . British Medical Journal , 23 October , 1982, Vol.285, p. 1185 – 1188.
19. Ulysses Fagundes-Neto , Isabel Cristina Affonso Scarletsky . The gut at war : the consequences of enteropathogenic Esch.coli infection as a factor of Diarrhoea and malnutrition , Sao Paulo Med J/ Rev. Paul Med. 2000 ; 118 (1) , p. 21 – 29
20. Enterobacterial infections . Encyclopedia of Childrens Health 2005 edition
21. Mercado E , Ochoa , T , Ecker L , ckary T. Fecal leucocytes in children infected with diarrhoeagenic Esch.coli Jour nal of clinical Microbiology 2011 ; 49 (4) : 1376 – 138122. Saxena SN , Mago ML , Rao Bhanu LN , Ahuja S, Gowal D “ Prevalence of multiple drug resistance amongst starins of Esch.coli isolates from cases of Diarrhoeaâ€, Indian J pediat , 1983, 50: p. 607 – 611.
23. Djie – Maletz A , Reither K. Donours High rate of resistance to locally used antibiotics among enteric bacteria from children in Northern Ghana. J Antimicrob chemother 2008 ; 61, 1315 – 1318.
24. Trung Vu N , Phung Van L , Chinh Huy L . Antibiotic Resistance in Diarrhoeagenic Esch.coli and shigella strains isolated from children in Hanoi , Vietnam . Antimicrob agents Chemother 2005 ; 49 (2) : 816 – 819.
25. Daniel R , Luciand R , Nanci S. Antibiotic for emperical treatment of acute infections diarrhoea in children Braz J In Fect Dis 2006 ; 10 (3)
26. Kyung Hee Kim, Inn-Soo such et al. Etiology of Childhood Diarrhoea in Korea . Jr.of Clinical microbiology , 1989; 1192 – 96.
27. Teresa Estrada-Garcia ,Jorge F, Cerna C. “ Drug-resistant Diarrhoeagenic Esch.coli , Maxico “, Emerging Infectious Diseases , August , 2005 ; P 1-5
28. Jav Sarantuya , Junichiro Nishi , Naoko Wakimoto et al .Typical Enteroaggregative Escherichia coli is the Most Pre valent pathotype among E.coli Strains Causing Diarrhoea in Mangolian Children “Journal of Clinical Microbiology , January , 2004 Vol, 42, No. 1 ; p.133 – 139.
29. Souman K , Bharwati S , Rajendran K. Virulance characteristics and Molecular Epidemiology of Enteroaggregative Esch.coli isolates from Hospitalised diarrhoeal patients in Kolkata , India . J clin Microbiol 2004 ; 42 (9).
30. Uber AP, Trabulsi LR , Irino K . Enteroaggregative Esch.coli from humans and animals differ in major phenotypic traits and virulence genes. FEMS Microbiology Letters 2006 ; 256 ( 2 ) : 251 – 7.
31. Andrej wein traub. Enteroaggregative Esch.coli Epidemiology , virulance and detection , Jornal of Medical Microbiology 2007 ; 56(1) : 4 – 8. Vaishnavi C , Kaur SS Beutin L Krueger W Phenotypic and molecular characterisation of clinically isolated Esch.coli Indian Journal of Pathology & Microbiology 2010 ; 53 (3) : 503 – 508.
32. Angela Cristina Rodrigues G . Production of cytolethal distending toxin and other virulence characteristics of Escherichia coli Strains of serogroup 086. Mem.Inst . Oswaldo Cruz 2001 ; 96 : 703 – 8 .
33. Vaishnavi C , Kaur S , Beutin L . Phenotypic and molecular Characterisation of clinically isolated Escherichia coli Indian Journal of Pathology & Microbiology 2010 ; 53 ( 3) : 503 – 508.
34. Priyanka S and Alka P . Isolation of Esch.coli , staphylococcus aureus and Listeria monocytogenes from milk products sold under market conditions at AGRA REGION , Acta agriculture Slovenic 2008 ; 92 (1) : 83 – 88.
35. Waldir P. Samar F , Cristiano G . Enteropathogenic Esch.coli strains among classical Enteropathogenic Esch.coli O serogroups Journal of clinical Microbiology 2002 ; 40 ( 9 ) : 3540 – 3541
36. Leila C.campos , Maria R franzolui , Leiz R Trabulsi “ Diarrhoeagenic Esch.coli categories among the Traditional Enteropathogenic Esch.coli O serogroups - A Review Mem Inst Oswaldo Cruz, Rio De Janeiro , October 2004 ; 99 ( 6) : 545 – 552.
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Published
2012-12-31
How to Cite
Tilak, G. P., & Mudaliar, J. lakshmi. (2012). Role Of Enteropathogenic Escherichia Coli In Paediatric Diarrhoeas In South India: Role Of Enteropathogenic Escherichia Coli In Paediatric Diarrhoeas. National Journal of Integrated Research in Medicine, 3(5), 21–26. Retrieved from https://www.nicpd.ac.in/ojs-/index.php/njirm/article/view/2094
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