Distribution Of Biofilm Producing Different Candida Species From Various Clinical Samples At A Tertiary Health Centre In Bihar
Distribution Of Biofilm Producing Different Candida Species From Various Clinical Samples At A Tertiary Health Centre In Bihar
Keywords:
Biofilm, Candida, fungi, Pathogenic, non-albicans CandidaAbstract
Objectives: Pathogenic fungi in the genus Candida can cause both superficial and serious systemic disease, and are now recognized as major agents of hospital-acquired infection. Many Candida infections involve the formation of biofilms on implanted devices. We undertook a prospective study to speciate the candida isolates, to detect biofilm formation among the isolates and to determine the predominant candida species in various clinical samples. Methods: This prospective, analytical study was done at IGIMS, Patna over a period of 2 months. The candida isolates were identified up to species level by standard mycological techniques like wet mount, KOH preparation and culture on Saboraud’s Dextrose agar (SDA). For speciation, rapid method – KB006Hi- Candida identification kit, CHROM agar and germ tube test were used. Result: A total of 40 non-repetitive isolates of Candida species from the clinical samples from both medical and surgical wards and ICU were included in this study. All patients on antifungal treatment were excluded. Out of the 40 cases isolated, 13 were C. albicans, 19 were C. tropicalis , 3 C. glabrata and 5 C. krusei .The maximum no. of candida isolated was from urine (16/40,40%) followed by foley’s catheter tip (10/40,25%) and endotracheal tube (8/40,20%). In our study non -albicans candida were more common than Candida albicans. Among non -albicans candida, Candida tropicalis (47.5%) was commonest.. Out of 40 candida species tested 8(20%) were found to be biofilm producers. Biofilm formation was more frequent among non- albicans species accounting for 6/8(75%) whereas C.albicans for 2/8(25%).Among biofilm producing non -albicans species C.tropicalis was the only biofilm producer. Conclusions: The isolation of non -albicans Candida (NAC spp). From clinical specimens should not be overlooked as these organisms are considered as emerging pathogens. Speciation is needed due to variation in species pathogenicity. Biofilm positivity was observed more with isolates from Foley’s catheter tip and endotracheal tube. [Shailesh K NJIRM 2016; 7(6): 106-109]
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